Day 2 of MSBM begun with back to back lectures from Prof. Joe Loo and Dr. Rachel Loo (UCLA), with Joe taking our students through myriad fragmentation techniques and Rachel introducing hybrid instruments. After a coffee break, we entered our first application lecture of the week by Dr. Harry Taylor (University of Nottingham), talking us through bottom-up proteomics and how to manually sequence peptides.
A long lunch break gave us ample opportunity to cool off with a quick dip in the sea at the local beach, Dance. The thunderstorm threatened on the forecasts kindly held off and we were treated to some more Croatian sunshine.
The first workshop of the week was led by Harry and Dave, with students implementing the skills they learnt in the morning lecture – taking a spectrum and manually sequencing an unknown peptide from it. Thank you to Ian (M4i, Maastricht) and Andreas (Shimadzu) for helping out with students.
A quick pit stop for coffee, and we were back underway, with Dr. Ian Anthony taking us through mass spectrometry data processing. Having spent the previous two hours manually sequencing a single spectrum, students looked at the importance of being able to automate their data processing in a new light.
The evening workshop saw students eagerly working their way through the exam paper, pizza, beer and wine to accompany them, along with a sprinkling of help from our faculty. Students worked long into evening, with many questions being worked through under torchlight!
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